TS No. 62: Spatial Pattern and Hotspot Analyses of Ganoderma Disease in Oil Palm Plantation Using the Geographical Information System

The growing demand for localized predictions, spatial pattern and hotspot analyses over large regions has prompted the application of the Geographical Information System (GIS) and geostatistics that can be used to analyse and manage plant disease information data. GIS has been used most extensively for mapping distributions of disease or specific genotypes of plant pathogen … Read more

TS No. 59: Cellular Localization of Transcripts via In situ RNA Hybridization

In situ hybridization is a widely used method that permits the localization of target mRNAs in a preserved tissue section. Most in situ hybridization methods use either non-isotopic or isotopically labelled riboprobes. The use of digoxigenin (DIG) compound is popular in the former type of labeling. For this, hybridized DIG-labelled ripoprobes are normally detected with high affinity anti-digoxigenin … Read more

TS No. 58: Plant Molecular Cytogenetic Analyses

Molecular cytogenetics involves a combination of molecular biology and cytogenetics. In general, this involves the use of a series of techniques referred to as the fluorescence in situ hybridization (FISH), in which DNA probes are labelled with differently coloured fluorescent tags to visualize one or more specific regions of the genome (Figure 1). The technology enables the … Read more

TS No. 57: Services for Genetic Transformation of Plants Species

Genetic engineering has been identified as a promising technology to further enhance the value of oil palm via the production of high value traits. Similarly, value can be generated for other plant services to ensure that the transfer of foreign gene(s) has taken place. Among the services are gene/DNA delivery, promoter analysis, and transient and … Read more

TT No. 411: Production of Pigeon Pea Integrated with Oil Palm

Pigeon pea (Cajanus cajan L.) is a leguminous shrub to a small tree and one of the main pulse crops. It ranks sixth in area and production among the grain legumes. Indigenous to India, it is now widely grown all over the subcontinent which produces almost 90% of the world’s crop. The plant requires a … Read more

TT No. 410: Integration of Yellow Cattle with Oil Palm

Optimizing land use in oil palm plantations is becoming increasingly important to maximize income from the crop. In 2000, MPOB introduced the integration of cattle in oil palm, and adoption of the practice has been good. In 2003, to upgrade the integration model, MPOB imported Yellow Cattle from China, a breed known for its hardiness … Read more

TT No. 409: Motorized Chisel (CkatTM) for Short Palm Harvesting

Harvesting is a very important activity in oil palm cultivation. Efficient harvesting ensures that high quality fresh fruit bunches (FFB) are sent to the mill quickly for processing to produce high quality oil with low free fatty acids (FFA). Efficient harvesting can be achieved by at least two factors; i.e. efficient harvesting tool and sufficient … Read more