The Internal Transcribed Spacer (ITS) region is the most widely sequenced DNA region in fungi by PRC analysis. The sequence comparison of the fungi ITS region is widely used in taxonomy and molecular phylogeny due to the high copy number of rRNA genes. Furthermore, it is easy to amplify even from small quantities of DNA and has a high degree of variation between closely related species. It is the most useful molecular systematics at the special level, and even within species. To be specific, the primer pair of ITS1+ITS4 has been used to identify the fungi species of ascomycetes, basidiomycetes, mastigomycetes and mitosporic fungi.

Main Research: Farah Nadia Omar