(MPOB Transfer of Technology)
For oil palm calli and suspension cultures, genetic variation analysis, estimation of ganome size and observation of nuclei activity is not possible via flow cytometry due to the low population of nuclei present. Hence, image cytometry or ICM method was developed to address this issue. The approach is to quantitate the chromogenic areas of the … Read more
The Internal Transcribed Spacer (ITS) region is the most widely sequenced DNA region in fungi by PRC analysis. The sequence comparison of the fungi ITS region is widely used in taxonomy and molecular phylogeny due to the high copy number of rRNA genes. Furthermore, it is easy to amplify even from small quantities of DNA … Read more
MPOB Microbial Identification System 1 (MIDS 1) utilises the Biolog which is a microbial identidication tool based on biochemical analyses. Biolog was developed in 1989 by Biolog, Inc. and well-known for its ability to identify and characteriste microorganisms. It is an identification system for bacterial, yeast and fungi using a microplate that analyse a microorganism … Read more
The study was conducted on 20 years old palms planted on coastal soil in Sepang, Selangor. One hundred and twenty infected palms were identify for this study. The palms were selected based on the presence of Ganoderma fructification at the based on the presence of Ganoderma fructification at the base with the palms still producing … Read more
The Ganoderma, the cause of basal stem rot (BSR) disease, is facultative basidiomycete fungus and a mahor pathogen of oil palm in Malaysia. One stratergy to manage BSR is by reducing chemical to infected stumps. Fu,ogation is one means of reducing inoculum f some root rotting fungi. Soil fumigants such as methylisothiocyanate (MITC), methyl bromide, … Read more