TS No. 59: Cellular Localization of Transcripts via In situ RNA Hybridization

In situ hybridization is a widely used method that permits the localization of target mRNAs in a preserved tissue section. Most in situ hybridization methods use either non-isotopic or isotopically labelled riboprobes. The use of digoxigenin (DIG) compound is popular in the former type of labeling. For this, hybridized DIG-labelled ripoprobes are normally detected with high affinity anti-digoxigenin … Read more

TS No. 58: Plant Molecular Cytogenetic Analyses

Molecular cytogenetics involves a combination of molecular biology and cytogenetics. In general, this involves the use of a series of techniques referred to as the fluorescence in situ hybridization (FISH), in which DNA probes are labelled with differently coloured fluorescent tags to visualize one or more specific regions of the genome (Figure 1). The technology enables the … Read more

TS No. 57: Services for Genetic Transformation of Plants Species

Genetic engineering has been identified as a promising technology to further enhance the value of oil palm via the production of high value traits. Similarly, value can be generated for other plant services to ensure that the transfer of foreign gene(s) has taken place. Among the services are gene/DNA delivery, promoter analysis, and transient and … Read more