MPOB has purchased a FACS Calibur Flow Cytometer (Becton Dickinson, USA) in 2003 (Figure 1). It is equipped with an argon ion laser for analysis of the fluorescent intensity of nuclei using the CellQuest software. Flow cytometry (FCM) is a rapid and convenient technique that allows accurate determination of (1) nuclear DNA content or genome size in plants, and (2) the ploidy level.

Main Research: Dr. Maria Madon