TS No. 21: Detection of Arsenic in Glycerine

Regularly drinking water high in arsenic results in chronic health problems. The Food Chemical Codex (FCC) and Cosmetics, Toiletry and Fragrance Association (CTFA) specify the maximum permissible limit of arsenic at 3 mg kg-1 while the United States Phamacopoeia (USP) at 1.5 mg kg-1. Main Research: Dr. Mohtar Yusof

TS No. 20: A Rapid Method for Determination of Oil Residue in Methyl Esters

Methyl esters are prepared via two routes, i.e. transesterification of vegetable oils with methanol or esterification of fatty acids with methanol. The former is the more common method, but the reaction may be incomplete leaving some residual oil with the methyl esters. This oil, or oil residue, should be monitored and preferably removed to produce high […]

TS No. 19: MPOB TAQ-Polymerase (M-Taq)

The polymerase chain reaction (PCR) has become the sine qua non of many molecular biology laboratories. It basically uses enzymes to mass replicate a portion of a deoxyribonucleic acid (DNA) strand for easier analysis, such as in searching for genes of interest. Like the nuclear chain reaction, PCR is an exponential process that continues as long […]

TS No. 18: Flow Cytometry Applications

MPOB has purchased a FACS Calibur Flow Cytometer (Becton Dickinson, USA) in 2003 (Figure 1). It is equipped with an argon ion laser for analysis of the fluorescent intensity of nuclei using the CellQuest software. Flow cytometry (FCM) is a rapid and convenient technique that allows accurate determination of (1) nuclear DNA content or genome […]

TT No. 344: Palm-based Polyurethane Flexible Slabstock Foams

Polyurethanes, or PU, constitute a group of polymers with highly versatile properties anda wide range of commercial applications. The material is used in almost every aspect of human life, in a multitude of applications in industries such as construction, automobile, refrigeration, furniture including mattresses, adhesives and sealants, footwear, ship building, transport, packaging, coating and paints. PU […]