TS No. 62: Spatial Pattern and Hotspot Analyses of Ganoderma Disease in Oil Palm Plantation Using the Geographical Information System

The growing demand for localized predictions, spatial pattern and hotspot analyses over large regions has prompted the application of the Geographical Information System (GIS) and geostatistics that can be used to analyse and manage plant disease information data. GIS has been used most extensively for mapping distributions of disease or specific genotypes of plant pathogen … Read more

TS No. 59: Cellular Localization of Transcripts via In situ RNA Hybridization

In situ hybridization is a widely used method that permits the localization of target mRNAs in a preserved tissue section. Most in situ hybridization methods use either non-isotopic or isotopically labelled riboprobes. The use of digoxigenin (DIG) compound is popular in the former type of labeling. For this, hybridized DIG-labelled ripoprobes are normally detected with high affinity anti-digoxigenin … Read more

TS No. 58: Plant Molecular Cytogenetic Analyses

Molecular cytogenetics involves a combination of molecular biology and cytogenetics. In general, this involves the use of a series of techniques referred to as the fluorescence in situ hybridization (FISH), in which DNA probes are labelled with differently coloured fluorescent tags to visualize one or more specific regions of the genome (Figure 1). The technology enables the … Read more

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