Fungi play important roles in soil structure, soil fertility and soil-plant interaction. Therefore, it is crucial to identify them. The FIDS 1 is a fungal identification system using ITS region which identifies a limited range of fungal species whereas, the FIDS 2 identified the fungal through LSU fragments with broader range of fungal species and the system is suitable for large number of samples. The FIDS 3 is a fungal identification system using 18s rDNA region. Sequence data from this region is widely used in molecular analysis for organisms evolutionary reconstruction, it slows evolutionary rate hence suitable for ancient divergences reconstruction. It is the most frequently used gene in phylogenetic studies and an important marker for random target polymerase chain reaction (PCR) in environmental biodiversity screening. The 18S rDNA-based approach is a useful tool for screening of fungal communities, and that they represent a more comprehensive picture of the community (Hunt et al., 2004). The fungal-specific primer pair of EF4/fung5 was developed for identification of the fungal diversity (Smit et al., 1999). This primer has been proven to identify the broader range of fungi species within Ascomycota, Basidiomycota, Zygomycota and Chytridiomycota (Smit et al., 1999; Borneman and Hartin, 2000; Hunt et al., 2004). 

Main Research: Hassriana Fazilla Sapri